Human IL12 kit HTRF®

Name: Human IL12 kit
Brand: Cisbio
SKU: 40425
Availability: InStock

Associated products

The HTRF human IL12 kit is designed for the quantification of IL12 release in cell supernatant.

No-wash
Low sample consumption
Highly specific

Download the protocols

The HTRF human IL12 kit is designed for the quantification of IL12 release in cell supernatant.

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Overview

Also known as NKSF (natural killer cell stimulatory factor) and CLMF (cytotoxic lymphocyte maturation factor), IL12 is produced by macrophages and B cells. Along with IFN gamma, IL12 promotes the differentiation of naïve T cells into TH1 cells, which in turn release IFNg and IL2. Because of its effects on TH1 induction, IL12 is strongly linked to autoimmunity. IL12 is a heterodimer of p35 and p4,0 which is shared with IL23. Cisbio's IL12 assay is specific for IL12p70 (full form) and does not cross react with IL23.

Assessment of serum samples often requires enhanced sensitivity. In some cases, AlphaLISA assays may have sufficient sensitivity to enable the detection of low levels of analytes in serum or plasma. Check out the Human IL-12 AlphaLISA kit’s analytical performances for more information or learn more about AlphaLISA. When assaying, always follow the recommended protocol and avoid highly haemolyzed samples.

Benefits

SCALABLE FROM 96 TO 1536WELLS
SPECIFIC OF IL12

Assay principle

Cell supernatant, sample, or standard is dispensed directly into the assay plate for the detection by HTRF® reagents (384-well low-volume white plate or Cisbio low-volume 96-well plate in 20 µl). The antibodies labeled with the HTRF donor and acceptor are pre-mixed and added in a single dispensing step, to further streamline the assay procedure. The assay can be run up to a 1536-well format by simply resizing each addition volume proportionally.

Assay data analysis

The 4 Parameter Logistic (4PL) curve is commonly recommended for fitting an ELISA standard curve. This regression enables the accurate measurement of an unknown sample across a wider range of concentrations than linear analysis, making it ideally suited to the analysis of biological systems like cytokine releases.

To fully understand how to deal with HTRF data processing and also 4PL 1/y² fitting, please visit this page.

Cisbio also worked with Myassays.com to help you in your data analysis. By clicking on this link, you’ll be able to access a free online software to run your IL12 analysis.

Technical specifications of human IL12 kit

Sample size	16 µL
Final assay volume	20 µL
Kit components	Lyophilized standard, frozen detection antibodies, buffers &protocol.
LOD &LOQ (in Diluent)	14 pg/mL &81 pg/mL
Range	81 13,000 pg/mL
Time to result	2h at RT
Calibration	NIBSC (95/544) value (IU/mL) = 0,01 x HTRF hIL12 value (pg/mL)
Species	Human only

Intra and inter assay

Intra-assay (n=24)

Sample	Mean [IL12] (pg/mL)	CV
1	200	6%
2	5000	4%
3	10000	3%
	Mean CV	4%

Each of the 3 samples was measured 24 times, and % CV was calculated for each sample.

Inter-assay (n=4)

Sample	[IL12] (pg/mL)	Mean (delta R)	CV
1	252	444	8%
2	1221	2158	11%
3	5909	10789	12%
		Mean CV	10%

Each of the samples was measured in 4 different experiments, and % CV was calculated for each sample.

Can HTRF technology answer to different scientific questions? An academic with industrial standards point of view

In collaboration with CNRS - Scientific Presentations

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Monitor immune regulation the simple way - Flyers

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Signaling in the Immune System - Brochures

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In collaboration with Blood assay solution - Application Notes

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One sample to measure them all - Posters

Guidelines from PBMC isolation to cytokine assay optimisation

Perform and optimize cytokine assays on PBMC - Technical Notes

How to freeze PBMC

Guideline for PBMC freezing - Technical Notes

How to measure multiple cytokines from 1 sample

What to expect at the bench - Videos

Product Insert IL12 (h) Kit / 62HIL12PEG-62HIL12PEH

62HIL12PEG-62HIL12PEH - Product Insert

HTRF makes cytokine detection easy

A fun video introducing you to cytokines assays with HTRF - Videos

HTRF Product Catalog

All your HTRF assays in one document! - Catalog

A guide to Homogeneous Time Resolved Fluorescence

General principles of HTRF - Guides

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This leaflet presents the analytical performances of each assay - Brochures

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Get the brochure about technology comparison. - Brochures

Safety Data Sheet (DEU) IL12 (h) Kit / 62HIL12PEG