Human IL23 kit HTRF®

Name: Human IL23 kit
Brand: Cisbio
SKU: 40435
Availability: InStock

Associated products

The HTRF human IL23 kit is designed for the quantification of human IL23 release in cell supernatant.

No-wash
Highly specific
Under 2h bench time

Download the protocols

The HTRF human IL23 kit is designed for the quantification of human IL23 release in cell supernatant.

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Overview

IL23 is part of the response against infection as it upregulates the expression of MMP9 (matrix metalloprotease 9), increases angiogenesis, and reduces CD8 T cell infiltration into tumors. In oncology and immuno-oncology, IL23 has been studied for its effects on T cell infiltration and its promotion on TH17 cell development. IL23 is a heterodimeric cytokine composed of p19 and p40 subunits, the latter being shared with IL12. Cisbio's Human IL23 assay is highly specific for IL23 and does not cross react with IL12.

Assessment of serum samples often requires enhanced sensitivity. In some cases, AlphaLISA assays may have sufficient sensitivity to enable the detection of low levels of analytes in serum or plasma. Check out the Human IL-23 alpha AlphaLISA kit’s analytical performances for more information or learn more about AlphaLISA. When assaying, always follow the recommended protocol and avoid highly haemolyzed samples.

Benefits

SPECIFIC FOR IL23
SCALABLE FROM 96 TO 1536

Assay principle

Cell supernatant, sample, or standard is dispensed directly into the assay plate for the detection by HTRF® reagents (384-well low-volume white plate or Cisbio low-volume 96-well plate in 20 µl). The antibodies labeled with the HTRF donor and acceptor are pre-mixed and added in a single dispensing step, to further streamline the assay procedure. The assay can be run up to a 1536-well format by simply resizing each addition volume proportionally.

Assay data analysis

The 4 Parameter Logistic (4PL) curve is commonly recommended for fitting an ELISA standard curve. This regression enables the accurate measurement of an unknown sample across a wider range of concentrations than linear analysis, making it ideally suited to the analysis of biological systems like cytokine releases.

To fully understand how to deal with HTRF data processing and also 4PL 1/y² fitting, please visit this page.

Cisbio also worked with Myassays.com to help you in your data analysis. By clicking on this link, you’ll be able to access a free online software to run your IL23 analysis.

Technical specifications of human IL23 kit

Sample size	16 µL
Final assay volume	20 µL
Kit components	Lyophilized standard, frozen detection antibodies, buffers &protocol.
LOD &LOQ (in Diluent)	30 pg/mL &92 pg/mL
Range	92 30,000 pg/mL
Time to result	3h at 4°C
Species	Human only

Intra and inter assay

Intra-assay (n=24)

Sample	Mean [IL23] (pg/mL)	CV
1	200	10%
2	10,000	2%
3	20,000	2%
	Mean CV	5%

Each of the 3 samples was measured 24 times, and % CV was calculated for each sample.

Inter-assay (n=4)

Sample	[IL23] (pg/mL)	Mean (delta R)	CV
1	307	259	12%
2	1920	1534	7%
3	12,000	8601	3%
		Mean CV	7%

Each of the samples was measured in 4 different experiments, and % CV was calculated for each sample

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In collaboration with CNRS - Scientific Presentations

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In collaboration with Blood assay solution - Application Notes

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One sample to measure them all - Posters

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Perform and optimize cytokine assays on PBMC - Technical Notes

How to freeze PBMC

Guideline for PBMC freezing - Technical Notes

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What to expect at the bench - Videos

Product Insert IL23 (h) Kit / 62HIL23PEG-62HIL23PEH

62HIL23PEG-62HIL23PEH - Product Insert

HTRF makes cytokine detection easy

A fun video introducing you to cytokines assays with HTRF - Videos

HTRF Product Catalog

All your HTRF assays in one document! - Catalog

A guide to Homogeneous Time Resolved Fluorescence

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Get the brochure about technology comparison. - Brochures

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A step by step protocol for Th17 cells differentiation from naive CD4 mouse cells - Technical Notes

Safety Data Sheet (DEU) IL23 (h) Kit / 62HIL23PEG