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SARS-Cov2 Spike S1 protein detection kit HTRF®

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  • Photo of SARS-COV2 SPIKE S1 KIT
  • Photo of SARS-COV2 SPIKE S1 KIT

This HTRF kit is designed to detect SARS-CoV-2 Spike S1 proteins in cell lysates or cell supernatant, as a marker of viral infection.

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  • No-wash No-wash
  • Low sample consumption Low sample consumption
  • High sensitivity High sensitivity
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This HTRF kit is designed to detect SARS-CoV-2 Spike S1 proteins in cell lysates or cell supernatant, as a marker of viral infection.

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Overview

The SARS-CoV-2 Spike S1 protein is one of two part of the virus Spike protein (S). The S1 subunit is responsible for the the virus interaction with host cells' transmembrane Angiotensin-converting enzyme 2 receptors (ACE2), while the S2 subunit is capable of conformational changes that allow the fusion of the viral particle envelope and host cell membrane upon S1/ACE2 binding. As such, the S1 protein is closely investigated for its role in viral infection and is reliable marker of infection as well as a potential vaccine immunogen target.

Detection of recombinant SARS-CoV-2 S1 protein

16 µL of recombinant protein in lysis buffer #5 were dispensed into a low volume white microplate before the addition of 4 µL of the premixed HTRF anti SARS-CoV2 capsid detection reagents. The HTRF signal was recorded after ON incubation.
SARS-CoV-2 nucleocapsid standard curve

standard curve Spike S1 kit

Sample size16 µL
Final assay volume20 µL
Time to resultsON at RT
Detection limit15 pg/mL
Dynamic range66 to 16,000 pg/mL
Sample compatibilityCell-based or cell supernatant
SARS-CoV2 Spike S1 standard curve
View product citations for Biomarkers 63ADK114PEG on CiteAb

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Product Insert Spike S1 protein kit / 63ADK114PEG-63ADK114PEH

63ADK114PEG-63ADK114PEH - Product Insert

Plate Reader Requirement

Choosing the right microplate reader ensures you’ll get an optimal readout. Discover our high performance reader, or verify if your lab equipment is going to be compatible with this detection technology.

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Guides

An innate and adaptive immunity recap