Home
In Vitro Diagnostics
Application Areas
Autoimmunity
Zinc transporter 8 autoantibody (ZnT8Ab) ELISA kit

Zinc transporter 8 autoantibody (ZnT8Ab) ELISA kit ELISA

The ZNT8/96 kit enables a quantitative determination in human serum of zinc transporter 8 autoantibodies associated with type I diabetes mellitus.

Download the protocols

The ZNT8/96 kit enables a quantitative determination in human serum of zinc transporter 8 autoantibodies associated with type I diabetes mellitus.

Size

Cat. number selected :

Quantity

Overview

Quantify ZnT8 autoantibodies specific to R 325 or to W 325 or to residue 325 non specific variants

Use as serological markers in type 1 diabetes mellitus

Benefits

ACCURATE AND RELIABLE ENZYMATIC ASSAY

Assay principle: sandwich

ZnT8 molecules are coated onto the ELISA plate wells. ZnT8-biotin complexes constitute the liquid phase. ZnT8-Ab from the sample bound to the ZnT8 coated onto the well will also interact with ZnT8-biotin to form a bridge. Addition of streptavidin peroxidase (SA-POD) detects the bound ZnT8-biotin. The unbound SA-POD in excess is washed off before the addition of the peroxidase substrate TMB.

Assay procedure: Overnight (16h-20h) at 2-8°C + 1 h at 2-8°C + 20 min RT / shaking + 20 min TA in the dark.